Title: Multiple transcription start sites and 5' alternate splicing of murine IL-3 receptor beta-chain transcripts
Authors: Norga, Koenraad ×
Pless, M
Stanulla, M
TePas, E C
Mathey-Prevot, B #
Issue Date: Nov-1994
Series Title: Biochemical and Biophysical Research Communications vol:205 issue:1 pages:886-92
Abstract: The murine interleukin-3 receptor beta-chain genes, IL-3R beta IL-3 and IL-3R beta c, encode the signal transducing chains of the high affinity receptors for IL-3 and IL-3, GM-CSF and IL-5 respectively. Little is known about the regulation of their expression. To enable the study of the promotors of IL-3R beta IL-3 and IL-3R beta c, we have characterized their respective 5' untranslated regions using a modified 5' RACE protocol. Four classes of alternatively spliced transcripts were isolated that initiate in a 400 nt region upstream from a previously reported start site(1). The initially reported partial IL-3R beta IL-3 clone belongs to the first class of transcripts(2). The second class starts in the middle of an intron as defined by the first class. The 3rd and 4th class establish 2 novel splice donor sites. These results were confirmed by RNAse-protection assay. The complex organization as evident from our data establishes an experimental framework for future experiments aimed at the study of the promoters for the murine IL-3R beta genes.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pediatric Hematology & Oncology Section (-)
× corresponding author
# (joint) last author

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