Global warming and pesticide pollution are major threats for aquatic biodiversity. Yet, how pesticideeffects are influenced by the increased frequency of extreme temperatures under global warming and howlocal thermal adaptation may mitigate these effects is unknown. We therefore investigated the combinedimpact of larval chlorpyrifos exposure, larval food stress and adult heat exposure on a set of fitness-relatedtraits in replicated low- and high-latitude populations of the damselfly Ischnura elegans. Larval pesticideexposure resulted in lighter adults with a higher water content, lower fat content, higher Hsp70 levelsand a lower immune function (PO activity). Heat exposure reduced water content, mass, fat contentand flying ability. Importantly, both stressors interacted across metamorphosis: adult heat exposurelowered the reduction of fat content, and generated a stronger decrease in PO activity in pesticide-exposed animals. Larval pesticide exposure and larval food stress also reduced the defense responseto the adult heat stress in terms of increased Hsp70 levels. In line with strong life history differencesin the unstressed control situation, high-latitude animals were less sensitive to food stress (body massand water content), but more sensitive to pesticide stress (development time and PO activity) and heatexposure (PO activity and Hsp70 levels). While low-latitude adults could better withstand the extremetemperature as suggested by the weaker increase in Hsp70, heat exposure similarly affected the delayedeffects of larval pesticide exposure at both latitudes. Our study highlighted two key findings relevant forecological risk assessment under global warming. Firstly, the delayed effects of larval pesticide exposureon adult damselflies depended upon subsequent adult heat exposure, indicating that larval pesticidestress and adult heat stress interacted across metamorphosis. Secondly, low- and high-latitude animalsresponded differently to the imposed stressors, highlighting that intraspecific evolution along naturalthermal gradients may shape sensitivity to pesticides.