This item still needs to be validated !
Title: A 68-kD antigen, which is probably a N-terminal fragment of the VLA-5 a5-subunit, is specific for differentiating keratinocytes
Authors: De Strooper, Bart
Wu, Rong-Rong
Jaspers, M
Van der Schueren, Bernadette
Vekemans, Sylvie
Carmeliet, Geert
Van Leuven, Freddy
van den Oord, Joost
Cassiman, Jean-Jacques #
Issue Date: 1996
Series Title: Dermatology (Basel, Switzerland) vol:193 issue:3 pages:212-20
Abstract: BACKGROUND: During terminal differentiation, basal keratinocytes lose gradually contact with the basement membrane, a process accompanied by the progressive functional down-regulation and loss of integrin expression. Understanding the molecular nature of this complex mechanism will eventually lead to insight into the pathogenesis of differentiation disorders of the epidermis, e.g. psoriasis. OBJECTIVE: The monoclonal antibody 8D9 against the very late antigen 5 (VLA-5) integrin subunit was used to study the expression and down-regulation of this protein in several experimental paradigms of keratinocyte differentiation. METHODS: Primary cultures of human keratinocytes were prepared and used as such, or after induction of terminal differentiation with methylcellulose and/or calcium. Expression of the 8D9 epitope was analyzed using immunoblotting, protein chemistry and immunocytochemistry on cultured cells and on skin biopsies from control and psoriatic patients. RESULTS AND CONCLUSION: The monoclonal antibody 8D9 reacts with the alpha 5-subunit of human VLA-5 integrin and with a 68-kD antigen that is strongly expressed in differentiating keratinocytes in vitro and in the cornified layers of human skin in vivo. Psoriatic skin showed additional immunoreactivity in the upper spinous and granular layers. Based on indirect immunological and chemical evidence we suggest that the 68-kD protein is an amino-terminal degradation product of the alpha 5-subunit, which provides a new and interesting marker of differentiating keratinocytes.
ISSN: 1018-8665
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Translational Cell & Tissue Research
Associated Laboratories - miscellaneous (-)
Clinical and Experimental Endocrinology
Human Mutations and Polymorphisms Section (-)
Forensic Biomedical Sciences
Department of Human Genetics - miscellaneous
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science