Title: Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris
Authors: Al Atalah, Bassam ×
Vanderschaeghe, Dieter
Bloch, Yehudi
Proost, Paul
Plas, Kirsten
Callewaert, Nico
Savvides, Savvas N
Van Damme, Els J M #
Issue Date: Apr-2014
Publisher: W. de Gruyter
Series Title: Biological Chemistry vol:395 issue:4 pages:413-24
Article number: 10.1515/hsz-2013-0267
Abstract: Abstract OrysaEULD1A is one of the five EUL genes in rice (Oryza sativa) encoding a putative carbohydrate-binding protein belonging to the family of Euonymus related lectins (EUL). The OrysaEULD1A sequence comprises two highly similar EUL domains (91% sequence similarity and 72% sequence identity) separated by a 23 amino acid linker sequence and preceded by a 19 amino acid N-terminal sequence. In the present study, the full-length protein OrysaEULD1A as well as its individual domains OrysaEULD1A domain 1 and 2 were expressed in Pichia pastoris. After purification of the recombinant proteins, their carbohydrate-binding specificity was analyzed and compared. Interestingly, all recombinant lectins showed clear specificity towards galactosylated structures. Furthermore, all recombinant proteins agglutinated red blood cells, indicating that the full-length protein OrysaEULD1A and its domains are true lectins. These results taken together with data previously reported for single-domain EUL proteins indicate that although the amino acids - responsible for the formation of the carbohydrate-binding site - are identical for all EUL proteins in rice, these lectins show different carbohydrate specificities. This promiscuity of the carbohydrate-binding site can be attributed to gene divergence.
ISSN: 1431-6730
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
× corresponding author
# (joint) last author

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