Title: Measurement of Intracellular Ca2+ Release in Intact and Permeabilized Cells Using 45Ca2+
Authors: Missiaen, Ludwig ×
Luyten, Tomas
Bultynck, Geert
Parys, Jan
De Smedt, Humbert #
Issue Date: 1-Mar-2014
Publisher: Cold Spring Harbor Laboratory
Series Title: Cold Spring Harbor Protocols vol:2014 issue:3
Article number: 10.1101/pdb.top066126
Abstract: Ca(2+) is an important ion that controls almost every function in a cell. Activator Ca(2+) can be released from intracellular Ca(2+) stores, and there are various ways to study this release. Here, we introduce a technique that uses radioactive (45)Ca(2+) to quantitatively measure the unidirectional release of Ca(2+) from the nonmitochondrial Ca(2+) stores in monolayers of cultured cells. This technique can be used in cells with an intact plasma membrane as well as in cells in which the plasma membrane has been permeabilized.
ISSN: 1559-6095
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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