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Title: Measurement of Intracellular Ca2+ Release in Permeabilized Cells Using 45Ca2+
Authors: Luyten, Tomas
Bultynck, Geert
Parys, Jan
De Smedt, Humbert
Missiaen, Ludwig # ×
Issue Date: 1-Mar-2014
Publisher: Cold Spring Harbor Laboratory
Series Title: Cold Spring Harbor Protocols vol:2014 issue:3
Article number: 10.1101/pdb.prot073189
Abstract: This protocol describes a technique to measure Ca(2+) release from the nonmitochondrial intracellular Ca(2+) stores in monolayers of saponin-permeabilized cells cultured in 12-well 4-cm(2) clusters. The (45)Ca(2+)-flux technique described here can only be applied to cell types that still adhere to the plastic after exposing them to saponin. We describe the permeabilization procedure, the loading of the nonmitochondrial Ca(2+) stores with (45)Ca(2+), and the subsequent (45)Ca(2+) efflux.
ISSN: 1559-6095
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular and Cellular Signaling
× corresponding author
# (joint) last author

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