Title: Comparative performances of HIV-1 RNA Load assays at low viral load levels: Results of an international collaboration
Authors: Swenson, Luke C ×
Cobb, Bryan
Geretti, Anna Maria
Harrigan, P Richard
Poljak, Mario
Seguin-Devaux, Carole
Verhofstede, Chris
Wirden, Marc
Amendola, Alessandra
Boni, Jurg
Bourlet, Thomas
Huder, Jon B
Karasi, Jean-Claude
Zidovec Lepej, Snjezana
Lunar, Maja M
Mukabayire, Odette
Schuurman, Rob
Tomazic, Janez
Van Laethem, Kristel
Vandekerckhove, Linos
Wensing, Annemarie M J
International Viral Load Assay Collaboration #
Issue Date: Feb-2014
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Clinical Microbiology vol:52 issue:2 pages:517-23
Article number: 10.1128/JCM.02461-13
Abstract: Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of ∼100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.
ISSN: 0095-1137
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

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