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Title: Detection of amplified sequences at 5q11-->q13 in a homogenously staining region found by fluorescent in situ hybridization in a case of B-cell non-Hodgkin's lymphoma
Authors: Wlodarska, Iwona
Peeters, Christiane
Dierick, H
Hilliker, Carl
Thomas, José
Mecucci, Christina
Cassiman, Jean-Jacques
Van den Berghe, Herman #
Issue Date: 1994
Series Title: Cytogenetics and cell genetics. vol:65 issue:3 pages:179-83
Abstract: Gene amplification is one of the molecular mechanisms possibly involved in the initiation of tumorigenesis. Homogenously staining regions and double minutes have been shown to contain amplified DNA sequences. We report here a case of B-cell non-Hodgkin's lymphoma (NHL) carrying an abnormal chromosome 5 with an hsr-like region at 5q11-->q13. Chromosome "painting" with a chromosome 5-specific library (pBS5) showed that the amplified material was indeed derived from chromosome 5. Further identification of the amplified DNA sequences was performed with a set of YAC probes localized to 5q12-->q14. The amplified region contained genetic loci defined by D5S6, D5S125, D5S112, and D5S39. A three-fold level of DNA amplification was visualized by Southern blotting using a DNA probe from the amplified region. The present results suggest that unknown gene(s) at 5q11-->q13 might be involved in the process of lymphomagenesis when amplification occurs.
ISSN: 0301-0171
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Genetics Section (-)
Department of Oncology - miscellaneous
Human Mutations and Polymorphisms Section (-)
Forensic Biomedical Sciences
Laboratory for Genetics of Malignant Disorders
Clinical Genetics
# (joint) last author

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