Title: Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light
Authors: Rocha, Susana
De Keersmaecker, Herlinde
Uji-I, Hiroshi
Hofkens, Johan
Mizuno, Hideaki # ×
Issue Date: 2014
Series Title: Methods in Molecular Biology vol:1076 pages:793-812
Article number: 10.1007/978-1-62703-649-8_36
Abstract: In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization microscopy, PALM) has been developed based on the photoswitchable fluorescent proteins. Here we introduce a basic protocol of PALM through the visualization of actin bundles with superresolution.
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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