Title: Viral vectors expressing a single microRNA-based short-hairpin RNA result in potent gene silencing in vitro and in vivo
Authors: Mendes Osório, Luísa Alexandra
Gijsbers, Rik
Oliveras Salvá, Marusela
Michiels, Annelies
Debyser, Zeger
Van Den Haute, Chris
Baekelandt, Veerle # ×
Issue Date: Nov-2013
Publisher: Elsevier BV
Series Title: Journal of Biotechnology vol:169 pages:71-81
Article number: S0168-1656(13)00487-2
Abstract: The characterization of RNA interference and the accompanying microRNAs (miRs), together with the exogenous expression of artificial miR-like elements, has led to the development of strategies for specific and potent gene silencing. In turn, this allows manipulation of gene expression levels for target validation purposes in cell culture or for the generation of animal models. In this study we determined the optimal strategy to achieve the most potent knockdown using miR-based viral vectors. We studied polycistronic miRs in a viral vector context and evaluated knockdown potency of multiple-miRs targeting the same seed sequence in parallel with miRs targeting different seed sequences, both for a reporter and endogenous mRNA targets. We demonstrate that potent knockdown can be obtained in vitro and in vivo using viral vectors that encode a single miR-based short hairpin RNA and report a generic and effective cloning platform for artificial miR30-based short-hairpin RNAs to generate potent knockdown viral vectors.
ISSN: 0168-1656
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group for Neurobiology and Gene Therapy
× corresponding author
# (joint) last author

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