Title: Evolutionary analysis of HBV "S" antigen genetic diversity in Iranian blood donors: A nationwide study
Authors: Pourkarim, MahmoudReza ×
Sharifi, Zohre
Soleimani, Ali
Amini-Bavil-Olyaee, Samad
Elsadek Fakhr, Ahmed
Sijmons, Steven
Vercauteren, Jurgen
Karimi, Gharib
Lemey, Philippe
Maes, Piet
Alavian, Seyed Moayed
Van Ranst, Marc #
Issue Date: Jan-2014
Publisher: Wiley-Liss
Series Title: Journal of Medical Virology vol:86 issue:1 pages:144-155
Article number: 10.1002/jmv.23798
Abstract: The genetic diversity of the HBV S gene has a significant impact on the prophylaxis and treatment of hepatitis B infection. The effect of selective pressure on this genetic alteration has not yet been studied in Iranian blood donors. To explore HBV evolution and to analyze the effects and patterns of hepatitis B surface antigen (HBsAg) mutations on blood screening assays, 358 Iranian blood donors diagnosed as asymptomatic HBV carriers were enrolled in this nationwide study. Large S and partial S genes were amplified and sequenced. HBV (sub) genotypes and synonymous and nonsynonymous mutations were investigated. The impact of naturally occurring mutations on HBsAg ELISA results was explored. Phylogenetic analyses revealed that isolated strains were of genotype D. The dominant subgenotype/subtype was D1/ayw2. Deletions and naturally occurring stop codons in the pre-S1 and major hydrophilic region (MHR) were identified. In total, 32.8% of the studied strains harbored 195 single or multiple mutations in the MHR, the majority of which were located at the first loop of the "a determinant" domain. The ayw2 subtype showed a significant effect on the ELISA signal/cut-off value and carried fewer mutations in the MHR. Nonsynonymous/synonymous substitution value indicated that negative selection was the dominant evolutionary force in the HBV S gene. This nationwide study revealed that mutation frequency of HBsAg among Iranian blood donors was much higher than previous reports from the different local regions. These findings regarding the significant differences in reactivity of ELISA among different subtypes of HBV and its correlation with the number of mutations at the MHR will be valuable to public health authorities. J. Med. Virol. XXXX:XX-XX (2013) © 2013 Wiley Periodicals, Inc.
ISSN: 0146-6615
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

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