Prolonged mechanical ventilation alters the expression pattern of angio-neogenetic factors in a pre-clinical rat model

Bruells, Christian S; Maes, Karen; Rossaint, Rolf; Thomas, Debby; Cielen, Nele; Bleilevens, Christian; Bergs, Ingmar; Loetscher, Ursina; Dreier, Agnes; Gayan-Ramirez, Ghislaine; Behnke, Brad J; Weis, Joachim

doi:10.1371/journal.pone.0070524

Prolonged mechanical ventilation alters the expression pattern of angio-neogenetic factors in a pre-clinical rat model

Author:

Bruells, Christian S

Maes, Karen ; Rossaint, Rolf ; Thomas, Debby ; Cielen, Nele ; Bleilevens, Christian ; Bergs, Ingmar ; Loetscher, Ursina ; Dreier, Agnes ; Gayan-Ramirez, Ghislaine ; Behnke, Brad J ; Weis, Joachim

Keywords:

Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, ENDOTHELIAL GROWTH-FACTOR, HYPOXIA-INDUCIBLE FACTOR, SKELETAL-MUSCLE, KAPPA-B, ANGIOPOIETIN-2 EXPRESSION, GENE-EXPRESSION, DIAPHRAGM, ANGIOGENESIS, DYSFUNCTION, RECEPTORS, Angiopoietin-2, Animals, Basic Helix-Loop-Helix Transcription Factors, Diaphragm, Down-Regulation, Gene Expression Regulation, Glucose Transporter Type 4, Hypoxia-Inducible Factor 1, alpha Subunit, Interleukin-1beta, Interleukin-6, Male, Muscle, Skeletal, RNA, Messenger, Rats, Rats, Wistar, Respiration, Artificial, Transforming Growth Factor alpha, Up-Regulation, Vascular Endothelial Growth Factor A, Ventilators, Mechanical, General Science & Technology

Abstract:

Mechanical ventilation (MV) is a life saving intervention for patients with respiratory failure. Even after 6 hours of MV, diaphragm atrophy and dysfunction (collectively referred to as ventilator-induced diaphragmatic dysfunction, VIDD) occurs in concert with a blunted blood flow and oxygen delivery. The regulation of hypoxia sensitive factors (i.e. hypoxia inducible factor 1α, 2α (HIF-1α,-2α), vascular endothelial growth factor (VEGF)) and angio-neogenetic factors (angiopoietin 1-3, Ang) might contribute to reactive and compensatory alterations in diaphragm muscle.