Title: Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves
Authors: Park, Jun-Gyu ×
Kim, Hyun-Jeong
Matthijnssens, Jelle
Alfajaro, Mia Madel
Kim, Deok-Song
Son, Kyu-Yeol
Kwon, Hyoung-Jun
Hosmillo, Myra
Ryu, Eun-Hye
Kim, Ji-Yun
Cena, Rohani B
Lee, Ju-Hwan
Kang, Mun-Il
Park, Sang-Ik
Cho, Kyoung-Oh #
Issue Date: Oct-2013
Publisher: Editions scientifiques Elsevier
Series Title: Veterinary Research vol:44 issue:1
Article number: 88
Abstract: Direct interspecies transmissions of group A rotaviruses (RVA) have been reported under natural conditions. However, the pathogenicity of RVA has never been directly compared in homologous and heterologous hosts. The bovine RVA/Cow-tc/KOR/K5/2004/G5P[7] strain, which was shown to possess a typical porcine-like genotype constellation similar to that of the G5P[7] prototype RVA/Pig-tc/USA/OSU/1977/G5P9[7] strain, was examined for its pathogenicity and compared with the porcine G5P[7] RVA/Pig-tc/KOR/K71/2006/G5P[7] strain possessing the same genotype constellation. The bovine K5 strain induced diarrhea and histopathological changes in the small intestine of piglets and calves, whereas the porcine K71 strain caused diarrhea and histopathological changes in the small intestine of piglets, but not in calves. Furthermore, the bovine K5 strain showed extra-intestinal tropisms in both piglets and calves, whereas the porcine K71 strain had extra-intestinal tropisms in piglets, but not in calves. Therefore, we performed comparative genomic analysis of the K71 and K5 RVA strains to determine whether specific mutations could be associated with these distinct clinical and pathological phenotypes. Full-length sequencing analyses for the 11 genomic segments for K71 and K5 revealed that these strains were genetically nearly identical to each other. Two nucleotide mutations were found in the 5[prime] untranslated region (UTR) of NSP5 and the 3[prime] UTR of NSP3, and eight amino acid mutations in VP1-VP4 and NSP2. Some of these mutations may be critical molecular determinants for RVA virulence and/or pathogenicity.
ISSN: 0928-4249
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Published article.pdf Published 4479KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science