Title: Screening of a synthetic peptide library against glycosomal phosphoglycerate kinase of Trypanosoma brucei
Authors: Samson, I ×
Van Aerschot, Arthur
Samyn, B
van Beeumen, J
Herdewijn, Piet #
Issue Date: Nov-1995
Publisher: Escom sci publ bv
Series Title: Letters in peptide science vol:2 issue:3-4 pages:217-219
Abstract: A synthetic peptide library composed of polystyrene beads was prepared employing the split-synthesis approach, using the 20 natural L-amino acids except for cysteine. This library was screened for interaction with glycosomal phosphoglycerate kinase (gPGK) of T. brucei, labelled with fluorescein or with biotin. Affinity beads were individually subjected to Edman microsequence analysis. The corresponding peptide sequences were synthesized as free peptide acids and evaluated for enzyme activity inhibition. The pentapeptide NWMMF was able to selectively inhibit gPGK with an IC50 of approximate to 80 mu M.
ISSN: 0929-5666
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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