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Title: Comparative evaluation of Tc-99m-ethylene bis-l-cysteine and Tc-99m-ethylene bis-l-beta-homocysteine during reversed phase HPLC analysis and electrophoresis at various pH conditions
Authors: Mang'era, KO ×
Tassaert, S
Cleynhens, Bernard
Verbruggen, Alfons
Verbeke, Kristin #
Issue Date: Mar-2001
Publisher: John wiley & sons ltd
Series Title: Journal of labelled compounds & radiopharmaceuticals vol:44 issue:4 pages:257-264
Abstract: Ethylene bis-L-beta -homocysteine (L,L-EH) differs from ethylene bis-L-cysteine (L,L-EC) in having an extra methylene group between each pair of amine and carboxyl groups. The objective of this study was to determine the effect of the extra methylene groups on the characteristics of the complex of these compounds with technetium-99m during analysis by reversed phase HPLC and by electrophoresis at various pH values. Up to pH 5.5, Tc-99m-L,L-EH exhibits a substantially longer retention time during reversed phase HPLC than Tc-99m-L,L-EC, suggesting a more lipophilic character for Tc-99m-L,L-EH under these conditions. On the other hand, Tc-99m-L,L-EH clearly possesses a higher negative charge in the pH range 3-6.5 as shown by the markedly greater migration towards the anode in electrophoresis experiments. A rational explanation for these seemingly opposing observations can not yet be offered.
URI: 
ISSN: 0362-4803
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiopharmacy
Translational Research in GastroIntestinal Disorders
× corresponding author
# (joint) last author

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