Title: Vascular endothelial growth factor-loaded injectable hydrogel enhances plasticity in the injured spinal cord
Authors: des Rieux, Anne ×
De Berdt, Pauline
Ansorena, Eduardo
Ucakar, Bernard
Damien, Jacobs
Schakman, Olivier
Audouard, Emilie
Bouzin, Caroline
Auhl, Dietmar
Simón-Yarza, Teresa
Feron, Olivier
Blanco-Prieto, Maria J
Carmeliet, Peter
Bailly, Christian
Clotman, Fréderic
Préat, Véronique #
Issue Date: Jul-2014
Publisher: John Wiley & Sons
Series Title: Journal of Biomedical Materials Research A vol:102 issue:7 pages:2345-55
Article number: 10.1002/jbm.a.34915
Abstract: We hypothesized that vascular endothelial growth factor (VEGF)-containing hydrogels that gelify in situ after injection into a traumatized spinal cord, could stimulate spinal cord regeneration. Injectable hydrogels composed of 0.5% Pronova UPMVG MVG alginate, supplemented or not with fibrinogen, were used. The addition of fibrinogen to alginate had no effect on cell proliferation in vitro but supported neurite growth ex vivo. When injected into a rat spinal cord in a hemisection model, alginate supplemented with fibrinogen was well tolerated. The release of VEGF that was incorporated into the hydrogel was influenced by the VEGF formulation [encapsulated in microspheres or in nanoparticles or in solution (free)]. A combination of free VEGF and VEGF-loaded nanoparticles was mixed with alginate:fibrinogen and injected into the lesion of the spinal cord. Four weeks post injection, angiogenesis and neurite growth were increased compared to hydrogel alone. The local delivery of VEGF by injectable alginate:fibrinogen-based hydrogel induced some plasticity in the injured spinal cord involving fiber growth into the lesion site. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A, 2013.
ISSN: 1549-3296
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science