Title: Separation of the anomers and isomers of 2'-deoxyuridine and thymidine by capillary zone electrophoresis
Authors: Van Schepdael, Ann ×
Vandewyer, M
Van Aerschot, Arthur
Herdewijn, Piet
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Oct-1993
Publisher: Elsevier science bv
Series Title: Journal of Chromatography vol:648 issue:1 pages:299-305
Abstract: Anomerisation and isomerisation take place during acidic degradation of 2'-deoxyuridine (dUrd) and thymidine (Thd) due to the opening and reclosure of the furanose ring. A capillary electrophoretic method was developed which was able to separate dUrd and Thd from their respective anomers and pentopyranosyl isomers. Using a fused-silica capillary (70 cm x 75 mum I.D.) the influence of the type, pH and concentration of the buffer was systematically investigated as well as the effect of voltage and temperature. With a 20 mM sodium tetraborate buffer of pH 9.5 and 10 for dUrd and Thd, respectively, good resolution between anomers and isomers was obtained at 20 kV and 50-degrees-C.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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