Journal of Chromatography vol:648 issue:1 pages:299-305
Anomerisation and isomerisation take place during acidic degradation of 2'-deoxyuridine (dUrd) and thymidine (Thd) due to the opening and reclosure of the furanose ring. A capillary electrophoretic method was developed which was able to separate dUrd and Thd from their respective anomers and pentopyranosyl isomers. Using a fused-silica capillary (70 cm x 75 mum I.D.) the influence of the type, pH and concentration of the buffer was systematically investigated as well as the effect of voltage and temperature. With a 20 mM sodium tetraborate buffer of pH 9.5 and 10 for dUrd and Thd, respectively, good resolution between anomers and isomers was obtained at 20 kV and 50-degrees-C.