Title: Early changes in the pupal transcriptome of the flesh fly Sarcophagha crassipalpis to parasitization by the ectoparasitic wasp, Nasonia vitripennis
Authors: Danneels, E.L. ×
Formesyn, E.M.
Hahn, D.A.
Denlinger, D.L.
Cardoen, Dries
Wenseleers, Tom
Schoofs, Liliane
de Graaf, D.C. #
Issue Date: 23-Oct-2013
Publisher: Pergamon Press
Series Title: Insect Biochemistry and Molecular Biology vol:43 issue:12 pages:1189-1200
Abstract: We investigated changes in the pupal transcriptome of the flesh fly Sarcophaga crassipalpis, 3 and 25 h after parasitization by the ectoparasitoid wasp, Nasonia vitripennis. These time points are prior to hatching of the wasp eggs, thus the results document host responses to venom injection, rather than feeding by the wasp larvae. Only a single gene appeared to be differentially expressed 3 h after parasitization. However, by 25 h, 128 genes were differentially expressed and expression patterns of a subsample of these genes were verified using RT-qPCR. Among the responsive genes were clusters of genes that altered the fly's metabolism, development, induced immune responses, elicited detoxification responses, and promoted programmed cell death. Envenomation thus clearly alters the metabolic landscape and developmental fate of the fly host prior to subsequent penetration of the pupal cuticle by the wasp larva. Overall, this study provides new insights into the specific action of ectoparasitoid venoms.
ISSN: 0965-1748
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
danneels et al 2013.pdf Published 962KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science