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Title: Foxp3(+) regulatory T cells are activated in spite of B7-CD28 and CD40-CD40L blockade
Authors: Vogel Geb.Zinsser, Isabel ×
Verbinnen, Bert
Maes, Wim
Boon, Louis
Van Gool, Stefaan
Ceuppens, Jan #
Issue Date: 2013
Publisher: Wiley-VCH
Series Title: European Journal of Immunology vol:43 issue:4 pages:1013-1023
Abstract: Costimulatory signals are required for priming and activation of naive T cells, while it is less clear how they contribute to induction of regulatory T (Treg)-cell activity. We previously reported that the blockade of the B7-CD28 and CD40L-CD40 interaction efficiently suppresses allogeneic T-cell activation in vivo. This was characterized by an initial rise in Foxp3(+) cells, followed by depletion of host-reactive T cells. To further investigate effects of costimulatory blockade on Treg cells, we used an in vitro model of allogeneic CD4(+) cell activation. When CTLA-4Ig and anti-CD40L mAb (MR1) were added to the cultures, T-cell proliferation and IL-2 production were strongly reduced. However, Foxp3(+) cells proliferated and acquired suppressive activity. They suppressed activation of syngeneic CD4(+) cells much more efficiently than did freshly isolated Treg cells. CD4(+) cells activated by allogeneic cells in the presence of MR1 and CTLA-4Ig were hyporesponsive on restimulation, but their response was restored to that of naive CD4(+) cells when Foxp3(+) Treg cells were removed. We conclude that natural Treg cells are less dependent on B7-CD28 or CD40-CD40L costimulation compared with Foxp3(-) T cells. Reduced costimulation therefore alters the balance between Teff and Treg-cell activation in favor of Treg-cell activity.
ISSN: 0014-2980
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Microbiology & Immunology - miscellaneous
Laboratory of Clinical Immunology
Laboratory of Pediatric Immunology
× corresponding author
# (joint) last author

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