Jpc-journal of planar chromatography-modern tlc vol:6 issue:4 pages:269-273
The corticosteroid hormones available on the European market may be identified by thin layer chromatography on silica gel. The use of different mobile phases, combined with detection by spraying with sulfuric acid in alcohol and heating, enables identification on the basis both of differences in R, and characteristic fluorescence at lambda = 365 nm. The salts are separated by development with 1-butanol - acetic acid - water (60 + 20 + 20, v/v). The alcohols, esters, and acetonides are identified by consecutive unidimensional development with two different mobile phases, dichloromethane - diethyl ether - methanol - water (77 + 15 + 8 + 1.2, v/v) followed by diethyl ether - toluene - n-butanol, saturated with water (80 + 15 + 5, v/v). Critical pairs with similar R(f) values, can be separated with acetic acid - carbon tetrachloride - n-heptane (40 + 30 + 30, v/v).