Title: An improved sample preparation for an LC method used in the age estimation based on aspartic acid racemization from human dentin
Authors: Yekkala, Raja ×
Meers, Carine
Hoogmartens, Jos
Lambrichts, Ivo
Willems, Guy
Van Schepdael, Ann #
Issue Date: Jan-2007
Publisher: Wiley-v c h verlag gmbh
Series Title: Journal of separation science vol:30 issue:1 pages:118-121
Abstract: The determination of age on the basis of aspartic acid (Asp) racemization in teeth is one of the most reliable and accurate methods to date. In this paper, the usefulness of HPLC coupled with fluorescence detection for determination of Asp racemization was evaluated. A modified sample preparation is proposed for better stability of o-phthaldialdehyde-N-acetyl-L-cysteine derivatives of D/L-Asp (due to the instability below pH 7). To ensure the accuracy of the method, the validation parameters' specificity, precision, linearity, and LOD were determined. Three dentin samples of premolar teeth, extracted from living individuals (bucco-lingual longitudinal sections of 1 mm thickness), were analyzed and quantitative results are discussed.
ISSN: 1615-9306
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
Forensic Dentistry
× corresponding author
# (joint) last author

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