Title: The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response
Authors: Simicek, Michal
Lievens, Sam
Laga, Mathias
Guzenko, Dmytro
Aushev, Vasily N
Kalev, Peter
Baietti, Maria Francesca
Strelkov, Sergei
Gevaert, Kris
Tavernier, Jan
Sablina, Anna # ×
Issue Date: Oct-2013
Publisher: Macmillan Magazines Ltd.
Series Title: Nature cell biology vol:15 issue:10 pages:1220-1230
Article number: 10.1038/ncb2847
Abstract: The RAS-like GTPase RALB mediates cellular responses to nutrient availability or viral infection by respectively engaging two components of the exocyst complex, EXO84 and SEC5. RALB employs SEC5 to trigger innate immunity signalling, whereas RALB-EXO84 interaction induces autophagocytosis. How this differential interaction is achieved molecularly by the RAL GTPase remains unknown. We found that whereas GTP binding turns on RALB activity, ubiquitylation of RALB at Lys 47 tunes its activity towards a particular effector. Specifically, ubiquitylation at Lys 47 sterically inhibits RALB binding to EXO84, while facilitating its interaction with SEC5. Double-stranded RNA promotes RALB ubiquitylation and SEC5-TBK1 complex formation. In contrast, nutrient starvation induces RALB deubiquitylation by accumulation and relocalization of the deubiquitylase USP33 to RALB-positive vesicles. Deubiquitylated RALB promotes the assembly of the RALB-EXO84-beclin-1 complexes driving autophagosome formation. Thus, ubiquitylation within the effector-binding domain provides the switch for the dual functions of RALB in autophagy and innate immune responses.
ISSN: 1465-7392
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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