Journal of Chromatography A vol:689 issue:2 pages:247-254
A liquid chromatographic method that can separate each of a series of diamino analogues of 2'- or 3'-deoxyadenosine from their main degradation product, adenine, is described. A PLRP-S 1000 Angstrom (8 mu m) 250 mm x 4.6 mm I.D. column was used at 60 degrees C. The method was developed by systematic evaluation of the influence of the mobile phase pH, the type and concentration of the organic modifier, the concentration of the ion-pairing agent and the buffer. The mobile phase consisted of tetrahydrofuran-0.2 M sodium octanesulphonate-0.2 M potassium phosphate buffer (pH 2.0)-water (15:25:30:30, v/v).