Title: Sample preparation for plasma measurement of plasminogen-activator inhibitor-1 antigen in large population studies
Authors: Macy, Em ×
Meilahn, En
Declerck, Paul
Tracy, Rp #
Issue Date: Jan-1993
Publisher: College amer pathologists
Series Title: Archives of Pathology & Laboratory Medicine vol:117 issue:1 pages:67-70
Abstract: Plasminogen activator inhibitor-1 is important in regulating fibrinolysis and may be an important cardiovascular risk factor. Because of this, there is increased interest in performing plasminogen activator inhibitor-1 assays in large epidemiologic studies. Our aim in this study was to determine the simplest blood collection methods that yield accurate results with our plasminogen activator inhibitor-1 antigen assay. Our results indicate that the following issues are important: (1) since there is a large circadian variation in plasminogen activator inhibitor-1 plasma levels, a target time frame must be established; (2) commercially available citrate collection tubes are adequate, if sample processing is rapid; (3) careful venipuncture is necessary, with freely flowing collection; hemolysis must be avoided; and (4) centrifugation of at least 30 000 g.min is required to avoid platelet contamination.
ISSN: 0003-9985
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Pharmaceutical Biology (-)
× corresponding author
# (joint) last author

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