Title: High throughput screening of yeast strains for desirable stress tolerant traits for bioethanol production
Authors: Mukherjee, Vaskar
Steensels, Jan
Van De Voorde, Ilse
Verplaetse, Alex
Aerts, Guido
Thevelein, Johan
Willems, Kris
Lievens, Bart
Verstrepen, Kevin
Ruyters, Stefan
Issue Date: Aug-2013
Publisher: John Wiley & Sons
Host Document: Yeast vol:30 issue:S1 (Article first published online: 22 AUG 2013) pages:S206
Conference: International Conference on Yeast Genetics and Molecular Biology edition:26 location:Frankfurt/Main, Germany date:29 August - 3 September 2013
Article number: PS16-6
Abstract: Implementation of very high gravity (VHG) fermentation technology in second generation bioethanol production using raw lignocellulosic biomass is fundamental to establish a commercially viable plant. However, so far the application of this technology is greatly restricted by the unavailability of a fermentative microorganism, resistant enough to the wide variety of stressors commonly encountered in VHG fermentation. In addition, the appropriate tools and knowledge to select such multi-stress tolerant microorganisms and to make a scientifically proven choice of the appropriate candidate strains have been lacking until recently. In this study we screened a large yeast culture collection, consisting of about 700 Saccharomyces cerevisiae and non-Saccharomyces strains from diverse origins, for different desirable traits for bioethanol production. These included, for example, osmotolerance, halotolerance, ethanol tolerance, thermotolerance, and tolerance against fermentation inhibitors like furfural and hydroxymethyl furfural as well as some heavy metals. To this end, a high throughput semi-automated robot was used for spotting up to 96 strains per screening plate. After incubation, plates were scanned and growth was recorded and analyzed using dedicated software. Cluster analysis showed clear differences in tolerance among species and among strains of the same species. In addition, strains showing co-tolerance against different traits could be identified. As such, our study enabled to efficiently select top candidate strains having desirable traits for VHG bioethanol production.
ISSN: 0749-503X
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Centre of Microbial and Plant Genetics
Microbial and Molecular Systems - miscellaneous
Molecular Microbiology and Biotechnology Section - miscellaneous (-)
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
Bioengineering Technology TC, Technology Campuses Ghent and Aalst

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