Title: Routine detection of buprenorphine in horse urine - possibilities and limitations of the combined use of radioimmunoassay, liquid-chromatography and gas-chromatography mass-spectrometry
Authors: Debrabandere, L ×
Van Boven, Maurits
Laruelle, L
Daenens, Paul #
Issue Date: Apr-1993
Publisher: Elsevier science bv
Series Title: Analytica chimica acta vol:275 issue:1-2 pages:295-303
Abstract: A radioimmunoassay developed for the determination of buprenorphine in human urine samples was examined for its applicability to screening post-race equine urine specimens. The detection limit of the immunoassay in equine urine is 0.1 ng ml-1. Confirmation methods using liquid chromatography with electrochemical detection and gas chromatography-mass spectrometry have detection limits of 0.2 ng ml-1 (unchanged buprenorphine and N-des-alkylbuprenorphine) and 0.1 ng ml-1 (after hydrolysis), respectively. The procedures were validated after in-field administration of 300 mug of buprenorphine to a test horse. The procedures were also applied to screening a large number of random post-race equine urine samples.
ISSN: 0003-2670
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Toxicology and Pharmacology
× corresponding author
# (joint) last author

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