Title: Liquid chromatography of gramicidin
Authors: Orwa, JA ×
Govaerts, Cindy
Roets, Eugene
Van Schepdael, Ann
Hoogmartens, Jos #
Issue Date: Jan-2001
Publisher: Vieweg
Series Title: Chromatographia vol:53 issue:1-2 pages:17-21
Abstract: A simple, selective method is described for separation of more than 10 gramicidin components on Spherisorb ODS B, 5 mum, 250 x 4.6 mm I. D. column, maintained at 50 degreesC. The mobile phase comprised methanol-water (71:29) at a flow rate of 1.0 mL min(-1). Detection was by UV at 282 nm. Valine gramicidins A, B and C were very well separated from the isoleucine gramicidins A, B and C. Four new gramicidin components were also resolved and their structures determined by liquid chromatography/mass spectrometry. The names 10-methionine valine gramicidin C, 4-methionine valine gramicidin A, valine gramicidin A hydroxypropyl and isoleucine gramicidin A hydroxypropyl were proposed. Robustness of the liquid chromatography method was evaluated by performing a full factorial design experiment. The method also showed good repeatability, linearity and sensitivity.
ISSN: 0009-5893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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