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Title: Influence of the incorporation of 1-(2,3-dideoxy-beta-D-erythro-hexopyranosyl)thymine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides
Authors: Augustyns, K ×
Van Aerschot, Arthur
Urbanke, C
Herdewijn, Piet #
Issue Date: Feb-1992
Publisher: Soc chimique belgique
Series Title: Bulletin des societes chimiques belges vol:101 issue:2 pages:119-130
Abstract: 1-(2,3-dideoxy-beta-D-erythro-hexopyranosyl)thymine was used at several positions as nucleoside substitute in the synthesis of oligonucleotides using the H-phosphonate chemistry. A 13-mer with one hexose nucleoside substitution at each end had equivalent hybridization properties as compared to the non-modified 13-mer. However, addition of a second analogue at each end or modification in the middle of a 13-mer caused a sharp decrease in melting temperature. Substitution at the 3'-end with two nucleoside analogues was necessary to withstand the action of snake venom phosphodiesterase.
ISSN: 0037-9646
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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