Title: The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration
Authors: Frittoli, Emanuela ×
Matteoli, Gianluca
Palamidessi, Andrea
Mazzini, Elisa
Maddaluno, Luigi
Disanza, Andrea
Yang, Changsong
Svitkina, Tatyana
Rescigno, Maria
Scita, Giorgio #
Issue Date: Sep-2011
Publisher: Cell Press
Series Title: Immunity vol:35 issue:3 pages:388-99
Article number: 10.1016/j.immuni.2011.07.007
Abstract: Dendritic cells (DCs) flexibly adapt to different microenvironments by using diverse migration strategies that are ultimately dependent on the dynamics and structural organization of the actin cytoskeleton. Here, we have shown that DCs require the actin capping activity of the signaling adaptor Eps8 to polarize and to form elongated migratory protrusions. DCs from Eps8-deficient mice are impaired in directional and chemotactic migration in 3D in vitro and are delayed in reaching the draining lymph node (DLN) in vivo after inflammatory challenge. Hence, Eps8-deficient mice are unable to mount a contact hypersensitivity response. We have also shown that the DC migratory defect is cell autonomous and that Eps8 is required for the proper architectural organization of the actin meshwork and dynamics of cell protrusions. Yet, Eps8 is not necessary for antigen uptake, processing, and presentation. Thus, we have identified Eps8 as a unique actin capping protein specifically required for DC migration.
ISSN: 1074-7613
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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