Title: Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma
Authors: Agnelli, Luca ×
Mereu, Elisabetta
Pellegrino, Elisa
Limongi, Tania
Kwee, Ivo
Bergaggio, Elisa
Ponzoni, Maurilio
Zamò, Alberto
Iqbal, Javeed
Piccaluga, Pier Paolo
Neri, Antonino
Chan, Wing C
Pileri, Stefano
Bertoni, Francesco
Inghirami, Giorgio
Piva, Roberto
Tousseyn, Thomas
European T-Cell Lymphoma Study #
Issue Date: Aug-2012
Publisher: W.B. Saunders
Series Title: Blood vol:120 issue:6 pages:1274-1281
Abstract: Anaplastic large-cell lymphomas (ALCLs) are a group of clinically and biologically heterogeneous diseases including the ALK(+) and ALK(-) systemic forms. Whereas ALK(+) ALCLs are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK(-) ALCL are missing, and their distinction from other T-cell non-Hodgkin lymphomas (T-NHLs) remains controversial. In the present study, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable of recognizing ALK(-) ALCL. Application of quantitative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded samples validated a 3-gene model (TNFRSF8, BATF3, and TMOD1) able to successfully separate ALK(-) ALCL from peripheral T-cell lymphoma not otherwise specified, with overall accuracy near 97%. In conclusion, our data justify the possibility of translating quantitative RT-PCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.
ISSN: 0006-4971
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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