Title: High speed HPLC determination of bis(pivaloyloxymethyl)-PMEA and its degradation products, mono(POM)-PMEA and PMEA
Authors: Augustijns, Patrick ×
Annaert, Pieter
Adriaens, S
De Clercq, Erik
Kinget, Renaat #
Issue Date: 1996
Publisher: Marcel dekker inc
Series Title: Journal of liquid chromatography & related technologies vol:19 issue:14 pages:2271-2283
Abstract: A high-speed HPLC gradient method for the determination of the antiviral ester prodrug bis(POM)-PMEA and its degradation products mono(POM)-PMEA and PMEA, is described. Perfusion chromatography enabled us to increase the flow rate up to 4 mL/min, resulting in high sample turn-over. Using a gradient system, the three compounds were separated from each other and the sample matrix within six minutes, while column equilibration was obtained in less than 3 minutes. Tetrabutylammonium was used as a counterion for PMEA. Notwithstanding the high flow rate, excellent reproducibility was obtained: the intraday as well as the interday precision, expressed as the relative standard deviation, for concentrations ranging from 5 to 50 mu M PMEA and bis(POM)-PMEA, was lower than 5%. The applicability of the method is demonstrated by studying the temperature- and pH-dependent degradation of bis(POM)-PMEA.
ISSN: 1082-6076
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Drug Delivery and Disposition
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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