Title: DNA-binding ligands from peptide libraries containing unnatural amino acids
Authors: Lescrinier, T
Hendrix, Christel
Kerremans, Lucien
Rozenski, Jef
Link, A
Samyn, P
Van Aerschot, Arthur
Lescrinier, Eveline
Eritja, R
Van Beeumen, J
Herdewijn, Piet # ×
Issue Date: Mar-1998
Publisher: Wiley-v c h verlag gmbh
Series Title: Chemistry - a European Journal vol:4 issue:3 pages:425-433
Abstract: An unnatural peptide-based library bound on a solid support, was screened for double-stranded-DNA (dsDNA)-binding ligands. For this purpose, fluorescein and rhodamine were used to label the single-stranded oligodeoxynucleotides, Beads containing products with affinity to dsDNA turned red in visible light and fluoresced yellow in UV light. A similar technique can be used for the selection of ligands which bind to a hairpin RNA, using a monolabelled oligoribonucleotide. The screening process revealed a high structure-affinity relationship in the successful products, Only six out of the twelve unnatural amino acids were selected, with the repeated appearance of AlaU, Sar and the secondary amino acids (Hyp, Inp). The affinity and selectivity for the target was determined using a DNase I protection assay.
ISSN: 0947-6539
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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