This item still needs to be validated !
Title: Plasminogen activator inhibitor 1 (PAI-1) in its active conformation: crystallization and preliminary X-ray diffraction data
Authors: Willems, PKA
Rabijns, Anja
Aertgeerts, K
Vleugels, Nele
Knockaert, I
De Bondt, HL
De Ranter, Camiel
Declerck, Paul # ×
Issue Date: Feb-1999
Publisher: Munksgaard int publ ltd
Series Title: Acta crystallographica section d-biological crystallography vol:55 pages:574-576
Abstract: Because of its intrinsic lability, wild-type plasminogen activator inhibitor 1 (PAI-1) cannot be crystallized in its active conformation. Therefore. a stable variant of PAT-I was used to retain the active conformation during crystallization. Four different crystallization conditions were evaluated in detail and two major types of crystals were detected. Whereas solutions consisting of either (i) cacodylate and sodium acetate, (ii) lithium sulfate and polyethylene glycol 4K, or (iii) imidazole, sodium chloride and sodium potassium phosphate: buffer revealed thin platelet crystals a solution (iv) containing ammonium acetate, citrate and polyethylene glycol JE; appeared to enhance the formation of clustered brush-like crystals. Crystals grown under condition (iii) were found to be suitable for X-ray data collection and consequent structural investigation. Data collection was 79.8% complete with a maximum resolution of 2.92 Angstrom. Importantly, PAI-1 retained its functional properties under all conditions.
ISSN: 1399-0047
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
Laboratory for Pharmaceutical Biology (-)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science