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Title: A renewable tissue resource of phenotypically stable, biologically and ethnically diverse, patient-derived human breast cancer xenograft models
Authors: Zhang, Xiaomei ×
Claerhout, Sofie
Prat, Aleix
Dobrolecki, Lacey E
Petrovic, Ivana
Lai, Qing
Landis, Melissa D
Wiechmann, Lisa
Schiff, Rachel
Giuliano, Mario
Wong, Helen
Fuqua, Suzanne W
Contreras, Alejandro
Gutierrez, Carolina
Huang, Jian
Mao, Sufeng
Pavlick, Anne C
Froehlich, Amber M
Wu, Meng-Fen
Tsimelzon, Anna
Hilsenbeck, Susan G
Chen, Edward S
Zuloaga, Pavel
Shaw, Chad A
Rimawi, Mothaffar F
Perou, Charles M
Mills, Gordon B
Chang, Jenny C
Lewis, Michael T #
Issue Date: Aug-2013
Publisher: Waverly Press
Series Title: Cancer Research vol:73 issue:15 pages:4885-97
Article number: 10.1158/0008-5472.CAN-12-4081
Abstract: Breast cancer research is hampered by difficulties in obtaining and studying primary human breast tissue, and by the lack of in vivo preclinical models that reflect patient tumor biology accurately. To overcome these limitations, we propagated a cohort of human breast tumors grown in the epithelium-free mammary fat pad of severe combined immunodeficient (SCID)/Beige and nonobese diabetic (NOD)/SCID/IL-2γ-receptor null (NSG) mice under a series of transplant conditions. Both models yielded stably transplantable xenografts at comparably high rates (∼21% and ∼19%, respectively). Of the conditions tested, xenograft take rate was highest in the presence of a low-dose estradiol pellet. Overall, 32 stably transplantable xenograft lines were established, representing 25 unique patients. Most tumors yielding xenografts were "triple-negative" [estrogen receptor (ER)-progesterone receptor (PR)-HER2+; n = 19]. However, we established lines from 3 ER-PR-HER2+ tumors, one ER+PR-HER2-, one ER+PR+HER2-, and one "triple-positive" (ER+PR+HER2+) tumor. Serially passaged xenografts show biologic consistency with the tumor of origin, are phenotypically stable across multiple transplant generations at the histologic, transcriptomic, proteomic, and genomic levels, and show comparable treatment responses as those observed clinically. Xenografts representing 12 patients, including 2 ER+ lines, showed metastasis to the mouse lung. These models thus serve as a renewable, quality-controlled tissue resource for preclinical studies investigating treatment response and metastasis. Cancer Res; 73(15); 4885-97. ©2013 AACR.
URI: 
ISSN: 0008-5472
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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