Title: Affinity modification of EcoRII DNA methyltransferase by the dialdehyde-substituted DNA duplexes: mapping the enzyme region that interacts with DNA
Authors: Gritsenko, OM ×
Koudan, EV
Mikhailov, SN
Ermolinsky, BS
Van Aerschot, Arthur
Herdewijn, Piet
Gromova, ES #
Issue Date: 2002
Publisher: Marcel dekker inc
Series Title: Nucleosides nucleotides & nucleic acids vol:21 issue:11-12 pages:753-764
Abstract: Affinity modification of EcoRII DNA methyltransferase (M-EcoRII) by DNA duplexes containing oxidized 2'-O-beta-D-ribofuranosylcytidine (Crib*) or 1-(beta-D-galactopyranosyl)thymine (Tgal*) residues was performed. Cross-linking yields do not change irrespective of whether active Crib* replaces an outer or an inner (target) deoxycytidine within the EcoRII recognition site. Chemical hydrolysis of M-EcoRII in the covalent cross-linked complex with the Tgal*-substituted DNA indicates the region Gly(268)-Met(391) of the methylase that is likely to interact with the DNA sugar-phosphate backbone. Both specific and non-specific DNA interact with the same M-EcoRII region. Our results support the theoretically predicted DNA binding region of M-EcoRII.
ISSN: 1525-7770
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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