Journal of liquid chromatography & related technologies vol:20 issue:2 pages:273-282
A method is described for separation of oxytetracycline and its related substances by capillary electrophoresis. Oxytetracycline is a broad spectrum antibiotic obtained by biosynthesis. Main related substances are 4-epioxytetracycline, alpha-apooxytetracycline, beta-apooxytetracycline, anhydrooxytetracycline, 2-acetyl-2-decarboxamidooxytetracycline tetracycline, 4-epitetracycline and terrinolide. Method development was undertaken with a mixture consisting of oxytetracycline and its related substances mentioned. Using an uncoated fused silica capillary, the influence of buffer pH and its concentration was systematically investigated. Non-ionic surfactant Triton X-100 was added to the running buffer for improving separation selectivity. Quantitative data are reported and compared with those of liquid chromatography and capillary zone electrophoresis.