Characterization of plasminogen activator inhibitor 1 mutants containing the P13 to P10 region of ovalbumin or antithrombin III: evidence that the P13 residue contributes significantly to the active to substrate transition
Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology vol:1387 issue:1-2 pages:291-297
The serpin plasminogen activator inhibitor 1 (PAI-I) can occur, in vitro, in both an inhibitory and a non-inhibitory but cleavable substrate form. In the present study, we have evaluated the effect of replacing the P13 to P10 region of PAI-1 (Val-Ala-Ser-Ser), with the P13 to P10 region of either the non-inhibitory serpin ovalbumin (Glu-Val-Val-Gly; PAI-l-ovalbumin) or the inhibitory serpin antithrombin III (Glu-Ala-Ala-Ala; PAI-l-antithrombin III). In addition, we have replaced Val at position P13 with Glu (PAI-1-P13 (Val --> Glu)). Wild-type (wt) PAI-1 revealed specific activities of 80 +/- 9% (mean +/- S.D., n=4) of the theoretical maximum value towards t-PA. PAI-l-ovalbumin, PAI-l-antithrombin III and PAI-1-P13 (Val-->Glu) revealed specific activities of 86+/-15%, 77+/-11%, and 100+/-30% respectively, towards t-PA and similar inhibitory properties towards u-PA. Surprisingly, upon inactivation at 37 degrees C, the active conformation of the PAI-1 mutants converted partly into a substrate conformation (i.e. 52+/-52%, 55+/-8.2% and 46+/-4.6% for PAI-I-ovalbumin, PAI-1-antithrombin III and PAI-1-P13 (Val - Glu), respectively) and partly into a latent conformation. This is in contrast to active wtPAI-1 which, as expected, is converted to the latent conformation (i.e. 86 +/- 1.0%), In conclusion, even though replacement of the P13 to P10 region of PAI-1 by the corresponding region of a non-inhibitory serpin or of an inhibitory serpin, does not directly affect its inhibitory properties, the nature of the amino acids in this region and of P13 in particular, contributes to its conformational transitions. (C) 1998 Elsevier Science B.V. All rights reserved.