Title: D-Altritol nucleic acids (ANA): Hybridisation properties, stability, and initial structural analysis
Authors: Allart, B ×
Khan, K
Rosemeyer, H
Schepers, G
Hendrix, Christel
Rothenbacher, K
Seela, F
Van Aerschot, Arthur
Herdewijn, Piet #
Issue Date: Jun-1999
Publisher: Wiley-v c h verlag gmbh
Series Title: Chemistry - a European Journal vol:5 issue:8 pages:2424-2431
Abstract: Oligonucleotides composed of a phosphorylated D-altritol backbone with nucleobases introduced in the 2'-position, hybridise strongly and sequence-selectively with RNA in an antiparallel way. The order of hybridisation strength is: dsANA > ANA:RNA > ANA:DNA. Complexes between ANA and RNA dr DNA are more stable than between HNA and natural oligonucleotides. The dsANA hybrid is extremely stable. When compared with an identical dsDNA sequence, the Delta T-m per modification is +10.2 degrees C for a hexamer. CD spectral analysis indicates that ANA complexes are very similar to the A-form dsRNA duplex. ANA are stable in alkaline medium up to pH 12 and, likewise, are not degraded in human serum.
ISSN: 0947-6539
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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