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Title: In vitro and in vivo investigation of the potential of Amorphous Microporous Silica as a protein delivery vehicle
Authors: Chaudhari, Amol ×
Van Mellaert, Lieve
Bauwens, Matthias
Vermaelen, Peter
Deroose, Christophe
Naert, Ignace
Vivan Cardoso, Marcio
Martens, Johan
Duyck, Joke #
Issue Date: 7-Aug-2013
Publisher: Hindawi Publishing Corporation
Series Title: BioMed Research International vol:2013 pages:1-10
Article number: Article ID 306418
Abstract: Delivering growth factors (GFs) at bone/implant interface needs to be optimized to achieve faster osseointegration. Amorphous microporous silica (AMS) has a potential to be used as a carrier and delivery platform for GFs. In this work, adsorption (loading) and release (delivery) mechanism of a model protein, bovine serum albumin (BSA), from AMS was investigated in vitro as well as in vivo. In general, strong BSA adsorption to AMS was observed. The interaction was stronger at lower pH owing to favorable electrostatic interaction. In vitro evaluation of BSA release revealed a peculiar release profile, involving a burst release followed by a 6 h period without appreciable BSA release and a further slower release later. Experimental data supporting this observation are discussed. Apart from understanding protein/biomaterial (BSA/AMS) interaction, determination of in vivo protein release is an essential aspect of the evaluation of a protein delivery system. In this regard micropositron emission tomography ( μ -PET) was used in an exploratory experiment to determine in vivo BSA release profile from AMS. Results suggest stronger in vivo retention of BSA when adsorbed on AMS. This study highlights the possible use of AMS as a controlled protein delivery platform which may facilitate osseointegration.
ISSN: 2314-6133
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Imaging & Pathology - miscellaneous
Nuclear Medicine & Molecular Imaging
Prosthetics
Centre for Surface Chemistry and Catalysis
× corresponding author
# (joint) last author

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