Title: Recognition of HNA and 1,5-anhydrohexitol nucleotides by DNA metabolizing enzymes
Authors: Vastmans, Karen
Rozenski, Jef
Van Aerschot, Arthur
Herdewijn, Piet # ×
Issue Date: May-2002
Publisher: Elsevier Biomedical Press
Series Title: Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology vol:1597 issue:1 pages:115-122
Abstract: Hexitol nucleic acids (HNA) as well as their 1,5-anhydrohexitol triphosphate building blocks were evaluated for their ability to be recognized by several DNA metabolizing enzymes. It was found that RNA polymerases can recognize the triphosphate of the adenine analogue. However, only the incorporation of a maximum of three consecutive building block analogues was possible under the applied experimental conditions. Terminal transferase was more successful succeeding in the elongation of a DNA primer with a maximum of 15 1.5-anhydrohexitol purine nucleotides. Furthermore, it was observed that the 1,5-anhydroaltritol triphosphate analogue of adenosine was a poor substrate for terminal transferase and that HNA could not act as a primer for this enzyme. Likewise, HNA did not function as a template for restriction enzymes, ligases or methylases. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 0167-4838
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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