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Title: Detailed Analysis of the Microbial Population in Malaysian Spontaneous Cocoa Pulp Fermentations Reveals a Core and Variable Microbiota
Authors: Meersman, Esther *
Steensels, Jan *
Mathawan, Melissa
Wittocx, Pieter-Jan
Saels, Veerle
Struyf, Nore
Bernaert, Herwig
Vrancken, Gino
Verstrepen, Kevin # ×
Issue Date: 16-Dec-2013
Publisher: Public Library of Sciene
Series Title: PLoS One vol:8 issue:12
Article number: e81559
Abstract: The fermentation of cocoa pulp is one of the few remaining large-scale spontaneous microbial processes in today’s food
industry. The microbiota involved in cocoa pulp fermentations is complex and variable, which leads to inconsistent production efficiency and cocoa quality. Despite intensive research in the field, a detailed and comprehensive analysis of the microbiota is still lacking, especially for the expanding Asian production region. Here, we report a large-scale, comprehensive analysis of four spontaneous Malaysian cocoa pulp fermentations across two time points in the harvest season and two fermentation methods. Our results show that the cocoa microbiota consists of a ‘‘core’’ and a ‘‘variable’’ part. The bacterial populations show a remarkable consistency, with only two dominant species, Lactobacillus fermentum and Acetobacter pasteurianus. The fungal diversity is much larger, with four dominant species occurring in all fermentations (‘‘core’’ yeasts), and a large number of yeasts that only occur in lower numbers and specific fermentations (‘‘variable’’ yeasts). Despite this diversity, a clear pattern emerges, with early dominance of apiculate yeasts and late dominance of Saccharomyces cerevisiae. Our results provide new insights into the microbial diversity in Malaysian cocoa pulp fermentations and pave the way for the selection of starter cultures to increase efficiency and consistency.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Centre for Food and Microbial Technology
* (joint) first author
× corresponding author
# (joint) last author

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