Title: Development of an improved liquid chromatographic method for the analysis of doxycycline
Authors: Yekkala, R ×
Diana, J
Adams, Erwin
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Sep-2003
Publisher: Vieweg
Series Title: Chromatographia vol:58 issue:5-6 pages:313-316
Abstract: An improved LC method is described for the separation of doxycycline and its impurities. The separation of 2-acetyl-2-decarboxamidodoxycycline from the main peak doxycycline is much better than that obtained with official phormacopoeia methods. The method is robust and shows good selectivity, repeatability, linearity and sensitivity. Eight commercial samples were examined using the method developed. The method uses an XTerra RP-18, 5 mum (25 cm x 4.6 mm I. D.) column kept at a temperature of 35 degreesC. UV detection is performed at 280 nm. The mobile phase consists of acetonitrile -0.2 M tetrabutylammonium hydrogen sulphate pH 7.0 - 0.3 M ethylenediaminetetraocetate pH 7.0 - water (130:350:350:170, v/v/v/v).
ISSN: 0009-5893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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