Title: Solution structure of a RNA decamer duplex, containing 9-[2-O-(beta-D-ribofuranosyl)-beta-D-ribofuranosyl]adenine, a special residue in lower eukaryotic initiator tRNAs
Authors: Luyten, Ingrid
Esnouf, RM
Mikhailov, SN
Efimtseva, EV
Michiels, P
Heus, HA
Hilbers, CW
Herdewijn, Piet # ×
Issue Date: 2000
Publisher: Wiley-v c h verlag gmbh
Series Title: Helvetica Chimica Acta vol:83 issue:6 pages:1278-1289
Abstract: The solution structure of the selt-complementary deca-ribenueleotide 5'-r(GCGA*AUUCGC)-3' containing 9-[2-O-(beta-D-ribofuranosyl)-beta-D-ribofuranosyl]adenine (A*),a modified nucleotide that occurs in lower eukaryotic methionine initiator tRNAs (tRNAs(i)(Met)),was determined by NMR spectroscopy. Unexpectedly, the modification has no effect on the thermal stability of the duplex. However, the extra ribose moiety is in the C(3')-endo conformation and takes up a well-defined position in the minor groove, which is in agreement with its position in tRNAs(i)(Met) as determined by X-ray crystallography. Molecular-dynamics simulations on the RNA duplex in H2O show that the position of the extra ribofuranose moiety seems to be stabilized by bridged H-bonds (mediated by two H2O molecules) to the backbone of the complementary chain.
ISSN: 0018-019X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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