Title: Biochemical-properties of natural and recombinant staphylokinase
Authors: Lijnen, Roger ×
Vanhoef, B
Vandenbossche, L
Collen, Desire #
Issue Date: Oct-1992
Publisher: Harcourt
Series Title: Fibrinolysis: An International Journal of Fibrinolysis and Thrombolysis vol:6 issue:4 pages:214-225
Abstract: The biochemical properties of natural staphylokinase (STAN), purified from the culture broth of a selected Staphylococcus aureus strain, were compared with those of different molecular species of recombinant staphylokinase (STAR), produced by expression of a genomic DNA fragment of the Staphylococcus aureus strain in E. coli. STAR was obtained from the supernatant (STAR-S) or periplasmic fraction (STAR-P) of the transformed E. coli either as a low molecular weight form lacking the 10 NH2-terminal amino acids (STA-DELTA10) (peak I following chromatography of STAR-S or STAR-P on CM-Sephadex, STA-CM-I) or as a mixture of mature STA (STA-M) and a proteolytic derivative lacking the 6 NH2-terminal amino acids (STA-DELTA6) (peak II following chromatography of STAR-S or STAR-P on CM-Sephadex, STA-CM-II), or it was obtained from the cytosol fraction (STAR-C) as STA-DELTA10.
ISSN: 0268-9499
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
× corresponding author
# (joint) last author

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