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Title: Induction of t-pa synthesis with intravenous bolus injection of vitamin-a palmitate in vitamin-a deficient rats
Authors: Bulens, F ×
Thompson, Ae
Stassen, Jm
Moreau, H
Declerck, Paul
Nelles, Lucien
Collen, Desire #
Issue Date: Oct-1992
Publisher: Harcourt
Series Title: Fibrinolysis: An International Journal of Fibrinolysis and Thrombolysis vol:6 issue:4 pages:243-249
Abstract: Retinoic acid and vitamin A palmitate induce tissue-type plasminogen activator (t-PA) synthesis in cultured human umbilical vein endothelial cells (HUVEC) in vitro without alteration of plasminogen activator inhibitor-1 (PAI-1) synthesis. Therefore the effect of intravenous bolus injection of water-miscible vitamin A palmitate on the blood fibrinolytic system was investigated in normal and vitamin A deficient rats. In 5 normal rats, injection of 200000 IU/kg of vitamin A palmitate did not induce a significant increase in euglobulin fibrinolytic activity, t-PA antigen and PAI activity in plasma nor of t-PA and PAI-1 mRNA in the heart within 240 min after injection. In groups of 3-6 vitamin A deficient rats, injection of 2000001U/kg of vitamin A palmitate induced a significant increase in t-PA antigen in plasma (1.9-fold after 60 min and 2.9-fold after 120 min), but no significant increase in plasma euglobulin fibrinolytic activity. A 2-fold increase in PAI activity was observed 90 min after injection of both vitamin A palmitate as well as of its solvent, suggesting that this induction was non-specific. The increase in t-PA antigen coincided with a significant increase in t-PA mRNA in heart tissue: 2.3-fold after 60 min and 4.3-fold after 120 min. PAI-1 mRNA in heart tissue increased 3.6-fold 90 min after injection with vitamin A palmitate but, surprisingly, not after injection of solvent.
ISSN: 0268-9499
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Pharmaceutical Biology (-)
Molecular and Vascular Biology
× corresponding author
# (joint) last author

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