Title: Separation of tetracycline and its potential impurities by liquid chromatography on XTerra RP-18
Authors: Capote, R ×
Diana, J
Roets, Eugene
Hoogmartens, Jos #
Issue Date: May-2002
Publisher: Wiley-v c h verlag gmbh
Series Title: Journal of separation science vol:25 issue:7 pages:399-404
Abstract: A linear gradient elution method is described for the separation of tetracycline, its fermentation impurities, and its degradation products. This method uses XTerra RP-18, 5 mum (25 cm x 4.6 mm ID), a silica-based stationary phase with reduced silanol activity. Gradient elution with mobile phases containing acetonitrile, 0.3 M tetrabutylammonium hydrogen sulfate pH 7.5, 0.3 M ethylenediaminetetraacetic acid pH 7.5, water was used at a flow rate of 1.0 mL/min. The column temperature was set at 40degreesC. The UV detection was performed at 280 nm. A central composite experimental design was performed to optimize the separation. The method permits a good separation of TC and its impurities. Some of these were not reported before and have now been identified for the first time. Some unidentified impurities were also separated.
ISSN: 1615-9306
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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