Collaborative study of the analysis of tylosin by liquid chromatography on wide-pore poly(styrene-divinylbenzene)
Paesen, J × Crommen, J Debeer, J Jin, Sh Porqueras, E Vanoverbeke, A Violon, C Hoogmartens, Jos #
Marcel dekker inc
Journal of liquid chromatography vol:18 issue:6 pages:1195-1205
A previously developed method for analysis of tylosin A and related substances by liquid chromatography using a wide-pore poly(styrene-divinylbenzene) stationary phase was examined in a multicentre study involving 7 laboratories and 3 samples. The main component and the impurities were determined. An analysis of variance showed absence of consistent laboratory bias and significant laboratory-sample interaction at the 1 % level. Estimates for the repeatability and reproducibility of the method, expressed as standard deviation (SD) of the result of the determination of tylosin A, were calculated to be 1.3 % and 1.6 % respectively.