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Title: Development and validation of an reversed-phase liquid chromatographic method for analysis of spiramycin and related substances
Authors: Chepkwony, HK ×
Vermaelen, Annick
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Jul-2001
Publisher: Vieweg
Series Title: Chromatographia vol:54 issue:1-2 pages:51-56
Abstract: A simple, robust, sensitive isocratic liquid chromatographic (LC) method suitable for the analysis of spiramycin is described. This method utilizes XTerra RP18 as the stationary phase and acetonitrile-0.2 M K2HPO4 (pH 6.5)-water, 39.5:5:55.5 (v/v), as mobile phase. The mobile phase flow rate is 1.0 mL min(-1); the column is maintained at 70 degreesC by immersion in a water-bath; and UV detection is performed at 232 nm. The method has good selectivity towards the major components (spiramycins I, II, and III), other related substances, and many impurities. The robustness of the method was evaluated by means of full-factorial experimental design.
ISSN: 0009-5893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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