Title: Evaluation of silanol-deactivated silica-based reversed phases for liquid chromatography of erythromycin
Authors: Paesen, J ×
Claeys, P
Roets, Eugene
Hoogmartens, Jos #
Issue Date: Feb-1993
Publisher: Elsevier science bv
Series Title: Journal of Chromatography vol:630 issue:1-2 pages:117-122
Abstract: The suitability of eleven silanol-deactivated reversed phases for the liquid chromatography of erythromycin was investigated. The selectivity and efficiency of each stationary phase were examined. The performance was compared to that of a non-deactivated C18 silica-based reversed-phase material, Hypersil C18 (5 mum). Two types of mobile phases were used, one containing no tetrabutylammonium (TBA) and the other containing TBA. Addition of TBA as a silanol-blocking agent improved the theoretical plate number and symmetry factor of the peaks corresponding to erythromycin A (EA) and erythromycin A enol ether for all the deactivated reversed phases. These results are an indication of the presence of some residual silanol activity in these phases. Separation of erythromycin E and EA was achieved on only two of the eleven phases. The selectivity was always poorer than that obtained in a previously described method using a poly(styrene-divinylbenzene) stationary phase.
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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