Title: Experimental design on liquid chromatographic parameters in the analysis of tetracycline on poly(styrene divinylbenzene)
Authors: De Beer, J ×
Hoogmartens, Jos #
Issue Date: Nov-1993
Publisher: Pergamon-elsevier science ltd
Series Title: Journal of pharmaceutical and biomedical analysis vol:11 issue:11-12 pages:1239-1250
Abstract: A previously described isocratic liquid chromatographic (LC) method for the analysis of tetracycline on poly(styrene-divinylbenzene) allows the complete separation and resolution of tetracycline (TC), 4-epiretracycline (ETC), anhydrotetracycline (ATC) and 4-epianhydrotetracycline (EATC). By means of a half-fraction factorial design, the importance of the individual chromatographic parameters and parameter interactions of this LC method was examined. The influence on the retention of ETC, TC and EATC is measured. A mathematical regression model was derived which predicts retention times with good reliability. Both the retentions of ETC and EATC are strongly affected by chromatographic parameters and parameter interactions, which only slightly affect the retention of TC. Adequate combination of the most relevant of these parameters enables optimization of the chromatographic separation if necessary.
ISSN: 0731-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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